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High Resolution Melt analysis


The PrimerDesign HRM learning zone

The PrimerDesign HRM learning zone is designed to give simple, well communicated information about high resolution melting analysis technology. Although the technology is relatively new the concepts are simple and using PrimerDesign products generating high quality HRM data is easy.

These resources are made freely available for to non-commercial entities for teaching and educational purposes. Please contact us is you would like to use them.

   
 
A beginner's guide to HRM - PDF format
 

PDF- click to download
   
 
Technical bulletins
 

Genotyping by simple HRM

Genotyping by raZor probe

Gene scanning by HRM

   
 
HRM educational flash animation
 
     
  Publications using HRM
 

Rouleau, E., C. Lefol, et al. (2009). "Quantitative PCR high-resolution melting (qPCR-HRM) curve analysis, a new approach to simultaneously screen point mutations and large rearrangements: application to MLH1 germline mutations in Lynch syndrome." Hum Mutat.

Pichler, M., M. Balic, et al. (2009). "Evaluation of High-Resolution Melting Analysis as a Diagnostic Tool to Detect the BRAF V600E Mutation in Colorectal Tumors." J Mol Diagn 11(2): 140-7.

De Leeneer, K., I. Coene, et al. (2008). "Rapid and Sensitive Detection of BRCA1/2 Mutations in a Diagnostic Setting: Comparison of Two High-Resolution Melting Platforms." Clin Chem.

Mackay J, Wright C and Bonfiglioli R. "A new approach to varietal identification in plants by microsatellite high resolution melting analysis: application to the verification of grapevine and olive cultivars." Plant Methods, 2008 4:8 ( 19 May 2008 )


Chateigner-Boutin, A. L. and I. Small (2007). "A rapid high-throughput method for the detection and quantification of RNA editing based on high-resolution melting of amplicons." Nucleic Acids Res 35(17): e114.


Grievink, H. and K. M. Stowell (2007). "Identification of ryanodine receptor 1 single-nucleotide polymorphisms by high-resolution melting using the LightCycler 480 System." Anal Biochem.
Dujols V, Kusukawa N, McKinney JT, Dobrowolsky SF, Wittwer CT. High-resolution melting analysis for scanning and genotyping., in Real Time PCR. Tevfik D, ed., Taylor and Francis, Abingdon, 2006.


Laurie, A. D., M. P. Smith, et al. (2007). "Detection of Factor VIII Gene Mutations by High-Resolution Melting Analysis." Clin Chem.


Reed GH, Wittwer CT. Sensitivity and specificity of single-nucleotide polymorphism scanning by high-resolution melting analysis. Clin Chem. 2004;50:1748-54.


Takano, E. A., G. Mitchell, et al. (2008). "Rapid detection of carriers with BRCA1 and BRCA2 mutations using high resolution melting analysis." BMC Cancer 8(1): 59.


Margraf, R. L., R. Mao, et al. (2008). "Rapid Diagnosis of MEN2B Using Unlabeled Probe Melting Analysis and the LightCycler 480 Instrument." J Mol Diagn 10(2): 123-8.


Bastien, R., T. B. Lewis, et al. (2008). "High-throughput amplicon scanning of the TP53 gene in breast cancer using high-resolution fluorescent melting curve analyses and automatic mutation calling." Hum Mutat.