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High Resolution Melt analysis


  Pre-validated HRM assays
 

Ready optimised High Resolution Melt assays for any mutation in any gene
 

How does the service work
"On demand" HRM assays for any mutations in any DNA sequence. Simply give us the details of your variation of interest and our expert team will professionally design the best possible primers that cater for your individual requirements. We then scientifically validate the assay on biologically derived gDNA in our own laboratory and package them for you at optimal concentrations for HRM analysis. The assays arrive with you in less than three weeks and no further optimisation by you is required.
Read comments on our services from our customers.

More information: Technical Bulletin

Kit guarantees
These kits are guaranteed to produce a high quality, specific, melting peak across the target variation. Due to the very small Tm shifts that some variations produce, it is not possible to guarantee that primer only kits will give rise to statistically valid genotyping data. For a guaranteed genotyping solution for all variations (including types 3 and 4) we recommend our raZor probe based assays.

Kit contents
Lyophilised pre-validated mix of primers, RNase/DNase free water, gDNA positive control.

Packaged, optimised and ready to use. Expect Better Data.

 

HRM assay

Unique product features:

  • Professional, expert design approach
  • Scientifically validated by World class scientists
  • Primer sequences supplied for publication (more details)

 

 
  Custom designed HRM assays
  Human DNA
HRM assays

Catalogue no: HRM-hu-600
Number of reactions
NCBI refSNP i.d

or


User supplied i.d.
genomic DNA sequence with variant


Handbook download: PDF qPCR

     
  Mouse DNA
HRM assays

Catalogue no: HRM-mo-600
Number of reactions
NCBI refSNP i.d

or


User supplied i.d.
genomic DNA sequence with variant

Handbook download: PDF qPCR

   
 

Rat DNA
HRM assays

Catalogue no: HRM-ra-600
Number of reactions
NCBI refSNP i.d

or


User supplied i.d.
genomic DNA sequence with variant


Handbook download: PDF qPCR

   
  Other species DNA
HRM assays

Catalogue no: HRM-all-600
Number of reactions
NCBI refSNP i.d

or


Species
Other species if not listed
User supplied i.d.
genomic DNA sequence with variant


Handbook download: PDF qPCR