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High Resolution Melt analysis


  Custom HRM assays with raZor probe
 

Razor sharp data guaranteed
 

How does the service work ?
"On demand" HRM raZor assays for any variations in any DNA sequence. Simply give us the details of your variation of interest and our expert team will professionally design the best possible primers and probe that cater for your individual requirements. We then scientifically validate the assay on biologically derived gDNA in our own laboratory and package them for you at optimal concentrations for HRM analysis. The assays arrive with you in less than three weeks and no further optimisation by you is required. When used with our Precision raZor Mix we guarantee the genotyping power of these assays.

 
 

What is an HRM raZor probe?
Custom designed HRM assays that come with a raZor probe are the highest specification HRM assay available. An especially modified DNA probe targets the site of the SNP or DNA variation of interest. HRM analysis of the probe/target melting adds huge power the quality of data achieved. We guarantee the genotyping typing power of these assays.

More information: Technical Bulletin

 
 

Kit guarantees
PrimerDesign offers this guaranteed genotyping solution for high resolution melting analysis. Our raZor probe assays (when used with our Precision HRM MasterMix) are 100% guaranteed to produce high quality genotyping data on a wide variety of real time PCR platforms, including these that are not specifically configured for HRM analysis.

Kit contents
Lyophilised pre-validated mix of primers and raZor probe, RNase/DNase free water, gDNA positive control.

Packaged, optimised and ready to use. Expect Better Data.

 

HRM assay

Unique product features:

  • raZor probe gives guaranteed SNP-typing power
  • Professional, expert design approach
  • Scientifically validated by World class scientists
  • Primer sequences supplied for publication (more details)

 

 
  Custom designed HRM assays with raZor probe
  Human DNA
HRM raZor assays

Catalogue no: HRM-Z-hu-600
Number of reactions
NCBI refSNP i.d

or


User supplied i.d.
genomic DNA sequence with variant


Handbook download: PDF qPCR

     
  Mouse DNA
HRM raZor assays

Catalogue no: HRM-Z-mo-600
Number of reactions
NCBI refSNP i.d

or


User supplied i.d.
genomic DNA sequence with variant

Handbook download: PDF qPCR

   
 

Rat DNA
HRM raZor assays

Catalogue no: HRM-Z-ra-600
Number of reactions
NCBI refSNP i.d

or


User supplied i.d.
genomic DNA sequence with variant


Handbook download: PDF qPCR

   
  Other species DNA
HRM raZor assays

Catalogue no: HRM-Z-all-600
Number of reactions
NCBI refSNP i.d

or


Species
Other species if not listed
User supplied i.d.
genomic DNA sequence with variant


Handbook download: PDF qPCR